Screening and Partial Purification of L-Asparaginase from the Bacteria Isolated from Soil Samples

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Screening and Partial Purification of L-Asparaginase from the Bacteria Isolated from Soil Samples

Enzymes are the selective proteins with high specificity that catalyze all the metabolic reactions in living organisms. Millions of enzymes are produced by prokaryotic and eukaryotic organisms. Use of microbial enzymes as drugs is an important research in the field of pharmaceutical industries. One of the most important discovery in this field is an enzyme L-asparaginase discovered by Clementi ...

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Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran

Introduction: The use of L-asparaginase as a chemotherapeutic agent is an effective strategy for the treatment of leukemic lymphoblasts. Since the use of bacterial L-asparaginase causes side effects due to hypersensitivity reactions, search for new enzyme sources is one of the active research areas. In this study, the characterization of produced L-asparaginase by indigenous yeast strains was i...

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Separation and purification of bacteria from soil.

Bacteria were released and separated from soil by a simple blending-centrifugation procedure. The percent yield of bacterial cells (microscopic counts) in the supernatants varied over a wide range depending on the soil type. The superantants contained large amounts of noncellular organic material and clay particles. Further purification of the bacterial cells was obtained by centrifugation in d...

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Exploration of the potential L-asparaginase producing bacteria from the soil of Gwalior (India)

Enzyme L-asparaginase (L-asparagines amidohydrolase EC 3.5.1.1) has the physiological function of hydrolyzing amide group of the side chain in Lasparagine to produce L-aspartate and ammonia. This spectacular property of the enzyme is utilized in chemotherapeutic treatment of certain kinds of lymphoblastic malignancies, mainly in acute lymphoblastic leukaemia (ALL) and lympho-sarcoma for nearly ...

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ژورنال

عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences

سال: 2017

ISSN: 2319-7692,2319-7706

DOI: 10.20546/ijcmas.2017.609.097